Anterograde Spread of Herpes Simplex Virus Type 1 Requires Glycoprotein E and Glycoprotein I but

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Our work raises a number of other questions. Nature 447: 326-30. SaHV-1 gH does not function with HSV-1 gL, gD, and gB, and HSV-1 gH does not function with SaHV-1 gL, gD, and gB. To characterize this further, we infected neurons with F-VP26/GFP and stained the cells with NC-1 antibodies and, simultaneously, with two VP5-specific MAbs. Target cells were transfected with a plasmid carrying luciferase under the control of the T7 promoter along with nectin-1 (B), CD155 (C), or empty vector. In order to follow cell-cell fusion events, cells were also stained with anti-gE antibody and a secondary Alexa Fluor 647 (far red)-conjugated antibody and with nuclear Hoechst 22358 stain to facilitate the detection of syncytia (Fig. 80, (7), 3592-3606 (2006).

The first mechanism involves HLA-II downregulation on infected cells through the combined actions of the virion host shutoff protein, γ34.5 protein and gB (74, 75). M.P.T. In this case, the directionality of spread in the nervous system may depend on factors other than intracellular transport, such as intrinsic immune responses and latency, as described in the following section. 7B). (G) Ensemble averages of gM-pHluorin (top, green line) and mCherry-tubulin (bottom, red line) relative fluorescence. J Virol 77: 11139–11149. Testing under experimental conditions showed that this vaccine was safe and induced protection against the clinical signs of field virus exposure within a week after vaccination [53], versus 2-3 weeks with a systemically administered vaccine [55].


Bars, 50 μm. Fruit bats that were seropositive for antibodies to FBAHV1 were identified from four species of the family Pteropodidae in different areas of Indonesia, although we must consider the possibility that this seropositivity may be due to immunological cross-reactivity between FBAHV1 and other, as yet unidentified, alphaherpesviruses. Tegument proteins could enter axons and be transported within vesicles, in association with capsids, with glycoproteins, or a combination of all three modes of targeting and transport. The Kuny et al. However, Ca2+ may also be released into the cytoplasm from other sources. This interference results from the cellular expression of gD, which interferes with alphaherpesvirus entry, probably by blocking ligand-binding sites of the gD receptors used for entry. Some herpesviruses, including FeHV-1, reactivate much more easily than others from the latent state, both under natural and experimental conditions.

In HCMV, the LTP (UL48), UL77 and UL93 (homologs of HSV-1 UL36, UL25, and UL17, respectively) as well as UL99, UL95, UL94, and UL71 (homologs of HSV-1 UL11, UL14, UL16, and UL51, respectively) are required for replication when the entire ORF is eliminated (Dunn et al., 2003). In contrast, Rab3a and Rab27a were not associated with exocytosis in PK15 cells (Fig. The internal primers used to mutate T150 to A and insert a novel silent AscI site for identification were T150Fasc (5′-CTTAAAAGGAGGCGCGCGGAAGAAGGCCCTACC-3′) and T150Rasc (5′-GGTAGGGCCTTCTTCCGCGCGCCTCCTTTTAAG-3′). Images of neuron cell bodies in the S compartment were taken at 8, 16, and 24 hpi, and representative sections at 24 hpi are shown in panel B (bars, 200 µm). We identify three types of gM-pHluorin fluorescence patterns: (1) gM-pHluorin dequenches and then rapidly diffuses into the surrounding plasma membrane. Expression of the heterologous VP22 protein was verified by fluorescence on infected cells and by immunoblotting with AGVO31, an anti-HSV-1 polyclonal antibody (9) (Fig. Louis, MO) to select for stable incorporation of pCC34.

In addition to gD, efficient EHV-1 entry also required the expression of glycosaminoglycans (GAGs), since CHO-K1 cells deficient in GAG synthesis (pgsA745) (10) were poorly infected with EHV-1 after a 2-h attachment phase (Fig. These examples support the contention that host gene expression can affect the progression of infection, with either beneficial or injurious effects for the host. In addition, since during infections ICP0 has been shown to lead to an increased number of ND10 domains that contain USP7 early in infection (24), we were interested in determining whether transfected ICP0 and its homologues had any effect on USP7 during transient transfection. The strategy was based on the striking result that despite injection directly into the brain, animals survived infection by the attenuated PRV vaccine strain Bartha but were killed by injection of the Ka strain of PRV (6). Renewed therapy with interferon alpha 2a successfully cleared up the inflammation. Matching secondary structural features resolved in the cryo-EM density with secondary structures predicted by sequence analysis identifies the triplex-binding region to be ORF32, a homolog of alphaherpesvirus UL17. This is the first report of a herpesvirus-associated disease in greater bilbies.

Similar but less-inhibitory effects of both IFNs were observed after direct infection of EC explants, being maximal when IFNs were added simultaneously or 6 h before HSV-1 infection. Many viruses target SGs for disruption and/or modification, including the alphaherpesvirus herpes simplex virus type 2 (HSV-2). The work has been published in PeerJ. Interaction of gH with α4β1-integrin results in the activation of PLC and generation of IP3 that binds to IP3R and mobilizes Ca2+ from the ER.