Cancer Gene Therapy – Bovine herpesvirus type 1 as a novel oncolytic virus

      Comments Off on Cancer Gene Therapy – Bovine herpesvirus type 1 as a novel oncolytic virus

Support for this prediction comes from the finding that type I interferon limits HSV-1 replication in cultured neurons (19) and small-animal models of infection (26, 57, 65). of BoHV-4 and cell death was examined by Wright’s nuclear staining with propidium iodide and by internucleosomal DNA fragmentation. PrV UL3.5 is required for virus egress. Bovine (Bos taurus) DNA extracted from calf thymus was purchased from Sigma. Secondary outbreaks last shorter amounts of time, but still suck ass. . Two of these glycoproteins, gE and gI, have been shown to be important for virulence and spread of several herpesviruses in all animal models tested (2, 9–11, 27, 33, 34, 39, 52, 55).

This gene is nonessential for virus replication, despite its contributing to posttranslational modification of virion proteins (18). We corrected a frameshift error in the published sequence and used the corrected sequence to design coterminal peptides from the C terminus. Recombinant viruses were propagated by infecting confluent monolayers of MDBK cells at a multiplicity of infection (m.o.i.) of 0.5 50% tissue culture infectious doses (TCID50) per cell and maintaining them in MEM with 10% FBS for 2 h. This gene is nonessential for virus replication, despite its contributing to posttranslational modification of virion proteins (18). As a result of alternative splicing of poly(A)+ LR-RNA in TG of infected calves (11, 12), ORF2 can be fused with ORF1 protein coding sequences (cDNA at 7 days postinfection [7 dpi cDNA]) or RF-B (15 dpi cDNA). Similar levels of viral DNA were detected in the tonsils of latently infected calves, regardless of which virus was used to infect the calves. VSV strain Indiana expressing GFP under the viral promoter (VSV-GFP) and the corresponding M protein mutant (VSVΔM51)4 were provided by Dr Brian Lichty (McMaster University).


CD4+ T cells are also the limiting cell type for antigen-induced proliferation in BHV-1 infection (7). The laboratory diagnosis of viral mastitis is laborious and expensive. BHV-1 is a member of the Herpesviridae family, in the Alphaherpesviridae subfamily. bICP0 transcription appears to be stimulated during reactivation from latency by cellular transcription factors that transactivate the bICP0 E promoter. The virus produced a febril response in 7 month old Holstein steers. Although the primary site of BHV-1 latency is sensory neurons, there is evidence that long-term persistence and reactivation occurs within germinal centers of pharyngeal tonsil (65). The dilutions yielding negative results were retested three times in order to confirm the absence of viral DNA.

DEX treatment of latently infected calves induces apoptosis of T cells that persist in trigeminal ganglia (TG) after infection (28). Soluble recombinant gp350 can also inhibit EBV infection of CD21-positive (CD21+) cells (36). The nature of cell death induced by BoHV-4 is highly controversial. Activation of CD4+ T helper lymphocytes requires specialized cells that process protein and present antigenic peptide fragments in the context of major histocompatibility complex class II (MHCII) molecules. They are required for the generation of antibody-producing cells (2), class II-restricted CD4+ cytotoxic T lymphocytes (CTL) (12,39), and NK-like cytotoxicity (9). Infection was performed with 1 TCID50/cell of a recombinant BoHV-4 expressing EGFP (BoHV-4EGFPΔTK) [ 3 ] and its effects were observed after 24, 48, 72, 96, 144, 216 hours post infection with an epi-fluorescence microscope (Leica). 75:8507-8515, 2001).

[21]). Additional studies provided evidence that ORF2 promotes the degradation of Notch3, but not that of Notch1, in a proteasome-dependent manner. The mRT-qPCR assay described here can detect viral co-infections both in technical validation experiments and in clinical samples. Sera were considered positive for BoHV-4 antibody when green fluorescence was detected at a dilution ≥ 1:40, with no fluorescence for uninfected cells at 1: 20. These results may suggest that the orientation of GVP 6/11a/16 in the membrane is such that GVP 11a is better exposed on the virion envelope and the cell surface than GVP 16. Several studies have demonstrated that the mature form of gB is heterodimeric and is generated by the presence of an internal putative proteasic site (RQKRS) in the second half of its aminoacidic sequence that is responsible for the cleavage of the two subunits, that are then covalently linked by disulfide bonds (Figure 1B) [3, 10]. In order to determine the extent of BoHV (-1 and/or -5) infections in bovines in this region of Brazil, 200 bovines were studied for the presence of BoHV DNA.

Homologs of UL3.5 are present in some alphaherpesviruses and have 20 to 30% overall amino acid homology that is concentrated in the N-terminal 50 amino acids. In vitro interaction of bovine herpesvirus 1 with uterine tube epithelial cells and oocytes. Two sensitive serum neutralization (SN) tests for the detection of antibodies to bovine herpesvirus-1 (BHV-1) in bovine sera were evaluated. Do cold sores, which are almost always caused by herpes simplex virus type 1 (HSV-1) , protect against genital herpes caused by herpes simplex virus type 2?