Epstein-Barr virus: the hematologic and oncologic consequences of virus-host interaction. – PubMed

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We conclude that the EBV vector system might offer an option for ex vivo manipulation of B cells and gene therapy of B-cell lymphomas. This review summarizes the role of EBV in malignancy and will focus on the latent proteins as a basis for understanding how EBV might contribute to the process of transformation. Many complications, including autoimmune hemolytic anemia, splenic rupture, hemophagocytic lymphohistiocytosis, and neurological complications have also been associated with infectious mononucleosis(1,5). In 54.9% of the cases, both sequences of HHV-6 and Epstein-Barr virus DNA were found, suggesting that a synergism of the two viruses may occur. A mature EBV viral particle has a diameter of approximately 120 nm to 180 nm. One may speculate that an increased load or altered presentation of a limited set of lytic or latent EBV proteins that cross-react with cellular antigens triggers and perpetuates the pathogenic processes that result in multiple sclerosis, systemic lupus erythematosus (SLE), and rheumatoid arthritis. There was no difference in the seropositive rates of the antibodies to EBV, cytomegalovirus, herpes simplex virus and herpes zoster virus.

The decade-long VZV vaccine trials in children with leukemia receive special emphasis because they have engendered considerable interest and debate. Instead, the virus can go “undercover” and live inside immune cells for years. While initially elevated four times above baseline, the transaminase levels returned to normal after three to seven days. Although humans are the only known natural host for EBV, EBV-like agents have been described in Old World nonhuman primates, including chimpanzee,15-17 baboon,16 18-21 African green monkey,22 gorilla,23 and macaque species.24-29 Although the relative prevalence of these viruses in animals in captivity or in the wild is unknown,30 several studies suggest that it may be as high as that in EBV in humans. These results demonstrate that EBV TK substrate specificity is narrower than those of alphaherpesvirus TKs and that thymidine analogs may be the most suitable nucleoside antivirals to target the enzyme. Here we report that the lack of 5-methylcytosine residues in CpG sites of virion DNA prevents the expression of essential lytic genes indispensable for viral DNA amplification during productive infection. In healthy EBV-seropositive persons, about 1 to 10 in 105 peripheral B cells are infected with EBV (14).

It is, however, clearly the predominant one (Fig. These are the only two vaccines in the world that prevent cancer. It’s actually when EBV is in its latent stage that the cancer begins to form in the nasopharynx, or the uppermost area of the throat located right behind the nasal cavity that it’s connected to. There are descriptions of EBV-associated lymphomas (4,5) localizing to breast and of bilateral breast cancer developing during the rare chronic active EBV infection syndrome (6). The strain used in this study, AD169, and the Towne strains were both used to develop vaccines [49]. Viral replication was also confirmed by the release of newly synthesized infectious viral particles in supernatants of EBV-infected monocytes. Reactivation and virus shedding occurs periodically throughout life.

Complementation of EBNA-2 deficiency by Rta depends on RBP-Jk and LMP-1, and Rta transactivation is required for optimal growth of KSHV+/EBV+ PEL lines. EBV transmission is almost exclusively via the salivary route, and virus can be recovered from throat washings of most seropositive individuals and IM patients (11). A repeated DNA sequence (the terminal repeat) present at the ends of the linear form (Figure 1) mediates circularisation in the infected cell so that latently infected cells contain the genome as a circular plasmid in the nucleus, with usually about 10 – 20 copies per infected cell. Successful infection and viral spread, within and between individual hosts, are necessary prerequisites for EBV pathogenesis. A region spanning amino acids 65 to 88 is required for the concentration of BFRF3 at a subnuclear site and the N-terminal 65 amino acids contain the sequences required for interaction with major capsid protein. High antibody titers against EBV-related proteins are not necessary for the diagnosis, because there can be a lack of serologic response in patients with CAEBV infection. 60(5), 476–482 (2007).

It is very rare for an individual to become re-infected with the virus. When infection with EBV occurs during adolescence, it causes infectious mononucleosis  – glandular fever – 35 to 50 percent of the time. Mao, J. The human EBV preferentially infects B cells, but occasionally infects other cell types, especially epithelial cells. Many bands apparently unrelated to EBV were detected. Herpes simplex virus type 2 (HSV-2), for example, replicates initially in the vagina and cervix and then takes up silent residence in sacral ganglia, whence it can reactivate and cause renewed viral replication and vaginal lesions. BBLF2/3 and its homologs in the other herpesviruses remain relatively poorly characterized.

After crossing the B-cell membrane, the virus enters cytoplasmic vesicles, where decapsidation takes place to allow transfer of the viral DNA to the cell nucleus.